Myosin heavy chain gene expression in mouse embryoid bodies. An in vitro developmental study.

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Myosin heavy chain gene expression in mouse embryoid bodies. An in vitro developmental study.

Embryoid bodies (EBs) are obtained when mouse pluripotential embryonic stem cells are grown in the absence of an embryonic fibroblast feeder layer. Seven- to 9-day-old EBs undergo rhythmical, spontaneous contractions and express the appropriate tissue- and developmental stage-specific cardiac and skeletal myosin heavy chain (MHC) genes. To study the expression patterns of these MHC genes in vit...

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Trim33 regulates early maturation of mouse embryoid bodies in vitro

Embryonic stem cells (ESCs) are an established model for investigating developmental processes, disease conditions, tissue regeneration and therapeutic targets. Previous studies have shown that tripartite motif-containing 33 protein (Trim33) functions as a chromatin reader during Nodal-induced mesoderm induction. Here we report that despite reduced proliferation, mouse ESCs deficient in Trim33 ...

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Myosin heavy chain gene expression in human heart failure.

Two isoforms of myosin heavy chain (MyHC), alpha and beta, exist in the mammalian ventricular myocardium, and their relative expression is correlated with the contractile velocity of cardiac muscle. Several pathologic stimuli can cause a shift in the MyHC composition of the rodent ventricle from alpha- to beta-MyHC. Given the potential physiological consequences of cardiac MyHC isoform shifts, ...

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Developmental regulation of myosin gene expression in mouse cardiac muscle

Expression of the two isoforms of cardiac myosin heavy chain (MHC), MHC alpha and MHC beta, in mammals is regulated postnatally by a variety of stimuli, including serum hormone levels. Less is known about the factors that regulate myosin gene expression in rapidly growing cardiac muscle in embryos. Using isoform-specific 35S-labeled cRNA probes corresponding to the two MHC genes and the two myo...

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Contractile protein content reflects myosin heavy-chain isoform gene expression.

Muscle fiber types are classified based on contractile speed and type of metabolism. Fast-contracting fibers involve mainly glycolytic-based metabolism, whereas slow-contracting fibers involve a more oxidative type of energy metabolism. The relationship between expression of the genes controlling these functional characteristics and their relative protein abundance in porcine muscle is unknown....

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1991

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)54589-0